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EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Reference
-999
-
more
2 and 5fold increases in kcat observed when BphI reaction is conducted in the presence of NAD+ and NADH
-999
-
more
value not detectable for R16A variant of BphI
-999
-
more
with 4-hydroxy-2-oxopentanoate at 50C turnover number is increased approximately 4fold
0.043
-
(4R)-4-hydroxy-2-oxopentanoate
L87W/Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
0.05
-
oxaloacetate
L87N variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
0.08
-
4-hydroxy-2-oxopentanoate
with Ca2+ chloride salt, pH 8.0, 25C
0.08
-
oxaloacetate
L87N/Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
0.089
-
(4S)-4-hydroxy-2-oxopentanoate
H20S variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
0.098
-
(4R)-4-hydroxy-2-oxopentanoate
L87N/Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
0.099
-
(4S)-4-hydroxy-2-oxopentanoate
H20A variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
Results 1 - 10 of 85 > >>