EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference  |
|---|
   1.13.11.50 | 8.5 |
- |
acetylacetate |
- |
657866 |
| 1.13.11.50 | 6.6 |
- |
Pentane-2,4-dione |
Strep-tagged enzyme |
671683 |
| 1.13.11.50 | 7 |
- |
Pentane-2,4-dione |
recombinant enzyme |
671683 |
| 1.13.11.50 | 8.5 |
- |
Pentane-2,4-dione |
native enzyme |
671683 |
| 1.13.11.50 | 0.00043 |
- |
4,4-difluoro-1-phenyl-1,3-butanedione |
apparent value at 25°C |
674150 |
| 1.13.11.50 | 0.0049 |
- |
1,1,1-trifluoro-2,4-pentanedione |
apparent value at 25°C |
674150 |
| 1.13.11.50 | 0.036 |
- |
1,1-difluoro-2,4-pentanedione |
apparent value at 25°C |
674150 |
| 1.13.11.50 | 6.5 |
- |
Pentane-2,4-dione |
apparent value at 25°C |
674150 |
| 1.13.11.50 | 0.0043 |
- |
1,1,1-trifluoro-pentane-2,4-dione |
native enzyme, using 0.09 mM 1,1,1-trifluoro-pentane-2,4-dione in 20 mM Tris/HCl buffer at pH 7.5 and 25°C |
675466 |
| 1.13.11.50 | 3.6 |
- |
Pentane-2,4-dione |
mutant enzyme E69Q, using 0.2 mM pentane-2,4-dione in 20 mM Tris/HCl buffer at pH 7.5 and 25°C |
675466 |
