EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
    1.13.11.27 | 6.8 |
- |
(4-hydroxyphenyl)-pyruvate |
- |
657978 |
| 1.13.11.27 | 0.065 |
- |
4-hydroxyphenylpyruvate |
activity is measured polarographically, assays include 0.5 microM of enzyme, 500 microM betaME, Fe2+ stoichiometric with enzyme, and 400 microM 4-hydroxyophenylpyruvate in 20 mM HEPES at pH 7.0 at 4°C |
672170 |
| 1.13.11.27 | 3.52 |
- |
4-hydroxyphenylpyruvate |
mutant A327R/L359M, pH 7, 25°C |
765506 |
| 1.13.11.27 | 1.4 |
- |
4-hydroxyphenylpyruvate |
mutant E12A, pH 6.5, 37°C |
765020 |
| 1.13.11.27 | 0.924 |
- |
4-hydroxyphenylpyruvate |
mutant F381A, pH 7, 25°C |
765756 |
| 1.13.11.27 | 4.96 |
- |
4-hydroxyphenylpyruvate |
mutant G411A, pH 7, 25°C |
765506 |
| 1.13.11.27 | 6.97 |
- |
4-hydroxyphenylpyruvate |
mutant G413W, pH 7, 25°C |
765506 |
| 1.13.11.27 | 0.7 |
- |
4-hydroxyphenylpyruvate |
mutant K10A, pH 6.5, 37°C |
765020 |
| 1.13.11.27 | 5.04 |
- |
4-hydroxyphenylpyruvate |
mutant L359M, pH 7, 25°C |
765506 |
| 1.13.11.27 | 0.7 |
- |
4-hydroxyphenylpyruvate |
mutant lacking 12 N-terminal residues, pH 6.5, 37°C |
765020 |
