EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
6.1.1.9 | -999 |
- |
more |
rate of hydrolysis of Thr-tRNAVal and Val-tRNAVal by wild-type and mutant enzyme, incorporation of amino acids valine and threonine into the enzyme |
650122 |
6.1.1.9 | 6.7 |
- |
tRNAVal |
wild-type enzyme, pH 7.7, 65°C |
653871 |
6.1.1.9 | 0.68 |
- |
L-valine |
with mutant tRNAVal with 3'-terminal 2-aminopurine base analogue, pH 7.5, 37°C |
653865 |
6.1.1.9 | 6.08 |
- |
L-valine |
with mutant tRNAVal with 3'-terminal 2-aminopurine base analogue, pH 7.5, 37°C |
653865 |
6.1.1.9 | 1.06 |
- |
L-valine |
with mutant tRNAVal with 3'-terminal inosine base analogue, pH 7.5, 37°C |
653865 |
6.1.1.9 | 6.08 |
- |
L-valine |
with mutant tRNAVal with 3'-terminal inosine base analogue, pH 7.5, 37°C |
653865 |
6.1.1.9 | 0.99 |
- |
L-valine |
with mutant tRNAVal with 3'-terminal isoguanosine base analogue, pH 7.5, 37°C |
653865 |
6.1.1.9 | 6.08 |
- |
L-valine |
with mutant tRNAVal with 3'-terminal isoguanosine base analogue, pH 7.5, 37°C |
653865 |
6.1.1.9 | 14.4 |
- |
L-valine |
with mutant tRNAVal with 3'-terminal purine riboside base analogue, pH 7.5, 37°C |
653865 |
6.1.1.9 | 13.9 |
- |
L-valine |
with wild-type tRNAVal, pH 7.5, 37°C |
653865 |