EC Number |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
---|
4.3.3.6 | 0.00055 |
- |
D-ribose 5-phosphate |
pyridoxal 5'-phosphate formation, wild-type protein, pH 7.5, 37°C |
726269 |
4.3.3.6 | 0.0006 |
- |
D-ribose 5-phosphate |
pyridoxal 5'-phosphate formation, His-tagged wild-type protein, pH 7.5, 37°C |
726269 |
4.3.3.6 | 0.00062 |
- |
D-ribose 5-phosphate |
pyridoxal 5'-phosphate formation, R288K mutant Pdx1 protein, pH 7.5, 37°C |
726269 |
4.3.3.6 | 0.00067 |
- |
D-ribose 5-phosphate |
pH 8.0, 37°C, free pyridoxal 5'-phosphate synthase subunit (Pdx1) |
715491 |
4.3.3.6 | 0.0007 |
- |
D-ribulose 5-phosphate |
pH 8.0, 37°C, free pyridoxal 5'-phosphate synthase subunit (Pdx1) |
715491 |
4.3.3.6 | 0.001 |
- |
L-glutamine |
pH 8.5, 37°C, glutaminase activity |
748203 |
4.3.3.6 | 0.004 |
- |
D-ribose 5-phosphate |
pH 8.0, 37°C, wild-type enzyme |
715491 |
4.3.3.6 | 0.022 |
- |
L-glutamine |
Pdx2 glutaminase activity, His-tagged H115A mutant Pdx1 protein, pH 7.5, 37°C |
726269 |
4.3.3.6 | 0.024 |
- |
L-glutamine |
Pdx2 glutaminase activity, His-tagged H115A/R138A mutant Pdx1 protein, pH 7.5, 37°C |
726269 |
4.3.3.6 | 0.024 |
- |
L-glutamine |
Pdx2 glutaminase activity, His-tagged K187A mutant Pdx1 protein, pH 7.5, 37°C |
726269 |