EC Number   |
Turnover Number Minimum [1/s] |
Turnover Number Maximum [1/s] |
Substrate |
Reference |
|---|
   4.1.3.43 | 0.1 |
- |
4-hydroxy-2-oxopentanoate |
G323L mutant, steady state, absence of BphJ cofactors, pH 8.0, 25°C |
721651 |
| 4.1.3.43 | 0.1 |
- |
propionaldehyde |
L87N/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C |
722458 |
| 4.1.3.43 | 0.11 |
- |
pyruvate |
L87N/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C |
722458 |
| 4.1.3.43 | 0.13 |
- |
4-hydroxy-2-oxopentanoate |
with Mg2+ chloride salt, pH 8.0, 25°C |
721659 |
| 4.1.3.43 | 0.13 |
- |
pentaldehyde |
L87A/L89A variant of BphI, app. kcat-value, pH 8.0, 25°C |
714244 |
| 4.1.3.43 | 0.131 |
- |
(4R)-4-hydroxy-2-oxopentanoate |
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C |
714244, 722458 |
| 4.1.3.43 | 0.132 |
- |
(4S)-4-hydroxy-2-oxopentanoate |
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25°C |
714244, 722458 |
| 4.1.3.43 | 0.14 |
- |
pentaldehyde |
Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C |
722458 |
| 4.1.3.43 | 0.14 |
- |
propionaldehyde |
L87W/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C |
722458 |
| 4.1.3.43 | 0.16 |
- |
Butyraldehyde |
Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25°C |
722458 |
