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EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Reference
3.6.1.17
23
-
Ap4A
wild-type enzyme
656173
3.6.1.17
26
-
Ap4A
mutant enzyme Y27A
656173
3.6.1.17
27
-
Ap4A
pH 7.8, 25°C
654817
3.6.1.17
27
-
diadenosine 5',5''''-P1,P4-tetraphosphate
in 50 mM Tris-HCl (pH 8.0), 5 mM MgCl2, 20 mM NaCl, 1 mM dithiothreitol, 0.2 mg/ml bovine serum albumin, at 37°C
719339
3.6.1.17
29
-
Ap4A
mutant enzyme Y27D
656173
3.6.1.17
30
-
Ap4A
mutant enzyme K81M
656173
3.6.1.17
31
-
Ap4A
mutant enzyme H38G
656173
3.6.1.17
31
-
Ap4A
mutant enzyme H38K
656173
3.6.1.17
40.8
-
Ap4A
pH 8.0, 25°C, wild-type enzyme
654489
3.6.1.17
43
-
5',5'-diadenosine tetraphosphate
pH 6.5, 1 mM Zn2+
687119
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