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EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Reference
0.1
-
4-hydroxy-2-oxopentanoate
G323L mutant, steady state, absence of BphJ cofactors, pH 8.0, 25C
0.1
-
propionaldehyde
L87N/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25C
0.11
-
pyruvate
L87N/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25C
0.13
-
4-hydroxy-2-oxopentanoate
with Mg2+ chloride salt, pH 8.0, 25C
0.13
-
pentaldehyde
L87A/L89A variant of BphI, app. kcat-value, pH 8.0, 25C
0.131
-
(4R)-4-hydroxy-2-oxopentanoate
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
0.132
-
(4S)-4-hydroxy-2-oxopentanoate
Y290F variant of BphI, steady-state kinetic parameter, pH 8.0, 25C
0.14
-
pentaldehyde
Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25C
0.14
-
propionaldehyde
L87W/Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25C
0.16
-
Butyraldehyde
Y290F variant of BphI, app. kcat-value, steady-state kinetic parameter, pH 8.0, 25C
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