EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
    4.2.1.75 | -999 |
- |
extremely heat labile |
5879 |
| 4.2.1.75 | -999 |
- |
more thermostable than enzymes from other sources |
5887 |
| 4.2.1.75 | -999 |
- |
purified enzyme shows remarkable thermostability, particularly when kept in phosphate buffer containing DTT and EDTA, indicating that the activity may depend on its oxidation state |
662062 |
| 4.2.1.75 | -999 |
- |
the enzyme is metathermostabile, irreversible denaturation process. At physiological temperature and in vitro, UROIIIS has a half-life time of 61.1 h, a long time for the enzyme to exert its function in the cell, dynamic behavior of the protein, three-state model, overview. Thermodynamic versus kinetic stability, overview |
713692 |
| 4.2.1.75 | -999 |
- |
UROIIIS is a thermolabile enzyme undergoing irreversible denaturation, unfolding kinetics of wild-type UROIIIS and mutants, circular dichroism, overview. A helical region in the molecule is essential to retain the kinetic stability of the folded conformation |
696334 |
| 4.2.1.75 | 20 |
- |
20% loss of activity in 10 min |
5888 |
| 4.2.1.75 | 30 |
- |
pH 7.4, t1/2: more than 14 days |
682774 |
| 4.2.1.75 | 30 |
- |
retention of 100% activity after 24 h |
5887 |
| 4.2.1.75 | 35 |
- |
enzyme reaction linear up to 30 min |
5882 |
| 4.2.1.75 | 37 |
- |
about 20% loss of activity after 48 h, about 50% loss of activity after 72 h, buffer containing 1 mM DTT |
662062 |
