EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
    3.5.4.12 | -999 |
- |
dCTP, and to a lesser extent dTTP, stabilizes against heat inactivation |
289004 |
| 3.5.4.12 | -999 |
- |
enzyme very heat-labile, stabilized against heat inactivation by dCTP, 0.125 mM and ethyleneglycol, 20% v/v |
289008 |
| 3.5.4.12 | -999 |
- |
enzyme very sensitive to preliminary heating, dCTP, 0.125 mM, and Mg2+, 2 mM, stabilizes against heat inactivation. Host-cell enzyme is more sensitive to heating than Herpes virus infected enzyme |
289007 |
| 3.5.4.12 | -999 |
- |
ethyleneglycol stabilizes against thermal and UV inactivation |
289006 |
| 3.5.4.12 | 0 |
- |
half-life: 30 min, Zn2+, thiols, 2-mercaptoethanol, and dCTP protect against inactivation |
289005 |
| 3.5.4.12 | 37 |
- |
enzyme from Herpes simplex virus infected BHK-21/C13 cells from baby-hamster is more resistant to heating at 37°C than enzyme from non-infected cells. In the presence of 0.125 mM dCTP and 2 mM Mg2+, 100% stable, 20 min at 37°C |
289007 |
| 3.5.4.12 | 43 |
- |
in the presence of ethyleneglycol: 100% stable |
289006 |
| 3.5.4.12 | 50 |
- |
enzyme very heat-labile, stabilized against heat inactivation by dCTP, 0.125 mM and Mg2+, or by increasing protein concentration |
289008 |
| 3.5.4.12 | 50 |
- |
ethyleneglycol stabilizes against thermal inactivation, when temperature increases to 50°C, leads to certain increase in specific activity |
289006 |
| 3.5.4.12 | 50 |
- |
thiol, 2-mercaptoethanol, dCTP and Zn2+ protect the enzyme against heat inactivation at 50°C |
289005 |
