EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
3.5.1.87 | 25 |
- |
activity is lost after 60 min at 25°C |
288946 |
3.5.1.87 | 25 |
55 |
no loss of activity after 6 d at 37°C, 50% loss of activity after 2 h at 50°C |
288948 |
3.5.1.87 | 30 |
35 |
no loss of activity after 30 min at pH 7.4, 30% activity lost after 30 min at 35°C |
288945 |
3.5.1.87 | 30 |
70 |
immobilized enzyme, in 100 mM borate-HCl, pH 8.0, 18 h, conversion rates decrease at temperatures over 55°C and are lost completely at over 70°C, for the immobilized enzyme system in production of optically pure L-amino acids, overview |
733195 |
3.5.1.87 | 45 |
- |
pH 8.0, the bienzymatic system retains almost total operability after 24 h of incubation at 45°C, overview |
735201 |
3.5.1.87 | 50 |
- |
purified enzyme, 60 min, about 86% activity remaining, half-life is 180 min |
733543 |
3.5.1.87 | 60 |
- |
the immobilized enzyme retains about 93% activity after 60 min, at pH 7.5, whereas the free enzyme loses about 50% activity |
733544 |
3.5.1.87 | 60 |
- |
the thermostability is significantly enhanced upon immobilization. The free enzyme experiences a decline in activity when the incubation temperature is increased from 50 to 60°C, the activity remains essentially unchanged for the immobilized enzyme. Even at higher temperatures the immobilized enzyme still displays higher thermostabilities than the free enzyme. Incubation at 80°C results in a complete loss of both, the free and immobilized enzyme |
713443 |
3.5.1.87 | 65 |
70 |
thermal stability studies performed on BsLcar show that maximum activity gradually decreases at temperatures over 65°C for 60 min, with 60% activity remaining after incubation at 70°C for 60 min |
711551 |
3.5.1.87 | 80 |
- |
incubation at 80°C results in a complete loss of both, the free and immobilized enzyme |
713443 |