EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
3.5.1.19 | -999 |
- |
ammonium sulfate at 1 M is able to produce a slight increase in its Tm (86.4°C) in comparison with that found in sodium phosphate pH 7.3. The nicotinamidase reaction products, nicotinic and pyrazinoic acids improved UbNic stability, increasing its Tm to 87.7°C and 87.1°C. Nicotinaldehyde and 5-bromonicotinaldehyde, increased Tm up to 92.7°C and 85.7°C |
755150 |
3.5.1.19 | 4 |
20 |
purified recombinant wild-type enzyme, 20 h, stable |
735084 |
3.5.1.19 | 25 |
70 |
- |
679806 |
3.5.1.19 | 37 |
- |
half-life: 2 h, stable in presence of 0.05 mM nicotinamide |
246569 |
3.5.1.19 | 37 |
- |
stable for more than 3 h |
246573 |
3.5.1.19 | 40 |
- |
pH 6.8, in presence of Co2+, 10 min, stable up to |
246588 |
3.5.1.19 | 50 |
- |
10 min, 27% loss of activity |
246573 |
3.5.1.19 | 50 |
- |
5 min, 50% loss of activity |
246569 |
3.5.1.19 | 55 |
- |
half-life: 2.5 min |
246576 |
3.5.1.19 | 60 |
- |
1 h, 5% loss of activity |
754017 |