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EC Number	Temperature Stability Minimum [�C]	Temperature Stability Maximum [�C]	Commentary	Reference
3.4.21.B55	-999	-	extra structural elements play a crucial role in adapting pyrolysin to hyperthermal environments	752548
3.4.21.B55	80	-	t1/2 is above 6000 min	722300
3.4.21.B55	95	-	half-life of wild-type enzyme: 12 h, half-life of mutant enzyme D818N/D820N is 18 h	724041
3.4.21.B55	95	-	half-life: 12 h (wild-type enzyme), 18 h (mutant enzymes R249E and D818N/D820N), 36 h (R249E/D818N/D820N)	752548
3.4.21.B55	95	-	t1/2: 540 min	722300
3.4.21.B55	100	-	half-life: 4 h	723099
3.4.21.B55	100	-	t1/2: 240 min	722300
3.4.21.B55	105	-	20 min, wild-type enzyme loses about 45% of its activity, mutant enzyme D818N/D820N loses about 30% of its activity	724041
3.4.21.B55	105	-	t1/2: 20 min	722300
3.4.21.B55	110	-	5 min, wild-type enzyme loses about 30% of its activity, mutant enzyme D818N/D820N loses about 20% of its activity	724041

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Release 2023.1 (January 2023)