EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
   3.4.21.66 | -999 |
- |
calcium ions contribute to the overall stability of the surface regions and improve the thermal stability of the enzyme |
29501 |
| 3.4.21.66 | -999 |
- |
irreversible inhibition by chloromethyl ketones causes marked stabilization against thermal denaturation |
29561 |
| 3.4.21.66 | -999 |
- |
unusually tight binding of Ca2+ by thermitase emerges as the most likely single influence responsible for its increased thermostability |
29553 |
| 3.4.21.66 | 50 |
- |
purified native enzyme, stable up to |
732254 |
| 3.4.21.66 | 50 |
- |
recombinant enzyme, stable up to |
732871 |
| 3.4.21.66 | 50 |
60 |
purified native enzyme, stable at |
753214 |
| 3.4.21.66 | 60 |
- |
denaturation above |
29549 |
| 3.4.21.66 | 70 |
- |
purified recombinant enzyme, substrate N-succinyl-Ala-Ala-Pro-Phe-4-nitroanilide, 20 h, 35% activity remaining |
731179 |
| 3.4.21.66 | 70 |
- |
quick inactivation above |
29565 |
| 3.4.21.66 | 80 |
100 |
thermostability profiles of the purified recombinant enzyme with different substrates, overview |
731179 |
