EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
   3.2.1.135 | -999 |
- |
EDTA stabilizes against thermal inactivation |
646810 |
| 3.2.1.135 | 20 |
60 |
C-terminal truncated enzyme ApuADELTA, 100% activity at 20-30°C, 50% at 45°C, pre-incubation at given temperatures for 1 h, pH 4.5, residual activity measured at 40°C, starch as substrate |
699051 |
| 3.2.1.135 | 20 |
60 |
full length enzyme, 100% activity at 20-30°C, 50% activity at 41°C, pre-incubation at given temperatures for 1 h, pH 4.5, residual activity measured at 40°C, starch as substrate |
699051 |
| 3.2.1.135 | 45 |
75 |
substrate pullulan, pre-incubation at 45, 50, 60, 65, 70, 75°C for 30, 60, and 120 min. about 80% activity after 120 min between 45 and 60°C, more than 60% activity after 120 min at 65°C, about 50% after 120 min at 70°C, around 20% activity after 120 min at 75°C |
696810 |
| 3.2.1.135 | 50 |
- |
30 min, pH 6.0, stable |
646822 |
| 3.2.1.135 | 50 |
- |
half-life 36 h |
646821 |
| 3.2.1.135 | 60 |
- |
- |
749679 |
| 3.2.1.135 | 60 |
- |
60 min, about 10% loss of activity |
646810 |
| 3.2.1.135 | 60 |
- |
half-life 24 h |
646821 |
| 3.2.1.135 | 60 |
70 |
the irreversible thermoinactivation of the enzyme is recorded in 50 mM sodium acetate, pH 5.5 at 60, 70 and 80°C. Pullulanase retains 85% of its activity after 50 min of incubation at 60°C, its residual activities at 70 and 80°C after 50 min are 70 and 15%, respectively |
703502 |
