EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
    2.7.4.14 | 4 |
- |
24 h, in absence of dithiothreitol the purified enzyme shows considerable loss of activity |
645147 |
| 2.7.4.14 | 4 |
- |
at pH 7 the enzyme is most stable when kept at 4°C |
645149 |
| 2.7.4.14 | 20 |
- |
in absence of glycerol, the half-life of a preparation with a specific activity of 80 is about 10 min, inactivation is partially reversed by addition of 2-mercaptoethanol |
645141 |
| 2.7.4.14 | 25 |
- |
30 min, stable |
645146 |
| 2.7.4.14 | 40 |
- |
30 min, enzyme form UMPK2 loses 70% of maximal activity, enzyme form UMPK1 loses 30% of its activity |
645146 |
| 2.7.4.14 | 45 |
- |
30 min, enzyme form UMPK2 loses more than 80% of maximal activity, enzyme form UMPK1 loses more than 60% of its activity |
645146 |
| 2.7.4.14 | 50 |
- |
30 min, enzyme forms UMPK2 and UMPK1 lose 90% of its activity |
645146 |
| 2.7.4.14 | 50 |
- |
midpoint denaturation temperature of the AUA chimeric enzyme |
645157 |
| 2.7.4.14 | 51 |
- |
midpoint denaturation temperature of UMP/CMP kinase |
645157 |
| 2.7.4.14 | 52 |
- |
midpoint denaturation temperature of the UAU chimeric enzyme |
645157 |
