EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
   2.7.1.63 | 28 |
- |
2 h, 30% loss of activity |
761859 |
| 2.7.1.63 | 30 |
40 |
purified recombinant enzyme, 100% remaining activity after 1 h |
721367 |
| 2.7.1.63 | 35 |
- |
2 h, 64% loss of activity |
761859 |
| 2.7.1.63 | 37 |
- |
24 h, pH 4-5: precipitation and 80% of activity retained, above pH 5.5: inactivation within 24 h, 0.3 M KCl stabilizes |
641743 |
| 2.7.1.63 | 40 |
- |
2 h, 95% loss of activity |
761859 |
| 2.7.1.63 | 40 |
- |
5 min, 50% loss of activity |
641754 |
| 2.7.1.63 | 50 |
- |
purified recombinant enzyme, 12% remaining activity after 1 h |
721367 |
| 2.7.1.63 | 55 |
- |
30 min, complete inactivation |
761859 |
| 2.7.1.63 | 55 |
- |
t1/2: 1.4 min (wild-type enzyme), t1/2: 3.2 min (mutant enzyme A231T), t1/2: 240 min (mutant enzyme F30L/T72A/A231T), t1/2: 2200 min (mutant enzyme F30L/T72A/V88M/A231T), t1/2: 10080 min (mutant enzyme F30L/R34P/T72A/V88M/A231T) |
760392 |
| 2.7.1.63 | 55.9 |
- |
Tm-value, wild-type enzyme |
760392 |
