Any feedback?
Please rate this page
(search_result.php)
(0/150)

BRENDA support

Refine search

Search Temperature Stability [°C]

show results
Don't show organism specific information (fast!)
Search organism in taxonomic tree (slow, choose "exact" as search mode, e.g. "mammalia" for rat,human,monkey,...)
(Not possible to combine with the first option)
Refine your search

Search term:

Results 1 - 9 of 9
EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Commentary Reference
Show all pathways known for 1.2.1.9Display the word mapDisplay the reaction diagram Show all sequences 1.2.1.9-999 - effect of NADP+ on the thermostability and the irreversible thermal denaturation of the nonphosphorylating glyceraldehyde-3-phosphate dehydrogenase from Streptococcus mutans. The enzyme-NADP+ binary complex shows a strongly decreased thermal stability, with a difference of about 20°C between the temperatures of the thermal transition peak maxima of the complex and the free protein. GAPN thermal inactivation is considerably decelerated in the presence of NADP+ showing that the apparent change in stability of the active centre can be the opposite to that of the whole protein molecule. NADP+ can also reactivate the inactive GAPN species, obtained by heating of the apoenzyme below the thermal denaturation transition temperature. The mechanism provides GAPN the sufficient flexibility for the earlier observed profound active site reorganizations required during the catalytic cycle. The elevated thermal stability of the apoenzyme may, in turn, be important for maintaining a constant level of active GAPN 741834
Show all pathways known for 1.2.1.9Display the word mapDisplay the reaction diagram Show all sequences 1.2.1.9-999 - hyperthermophilic 288347
Show all pathways known for 1.2.1.9Display the word mapDisplay the reaction diagram Show all sequences 1.2.1.9-999 - phosphate binds to the enzyme, resulting in the formation of a GAPN-phosphate binary complex characterized by a strongly decreased thermal stability, with a difference of at least 15°C between the maximum temperature of the thermal transition peaks, phosphate binds to the substrate C-3 subsite. Glycerol-3-phosphate has similar effects in thermal stability 656283
Show all pathways known for 1.2.1.9Display the word mapDisplay the reaction diagram Show all sequences 1.2.1.945 - 10 min, inactivation above 656781
Show all pathways known for 1.2.1.9Display the word mapDisplay the reaction diagram Show all sequences 1.2.1.945 - inactivation above 288338
Show all pathways known for 1.2.1.9Display the word mapDisplay the reaction diagram Show all sequences 1.2.1.960 - 6 min, 89% inactivation 288341
Show all pathways known for 1.2.1.9Display the word mapDisplay the reaction diagram Show all sequences 1.2.1.970 - 10 min, inactivation above 657250
Show all pathways known for 1.2.1.9Display the word mapDisplay the reaction diagram Show all sequences 1.2.1.975 - 10 min, complete inactivation 656781
Show all pathways known for 1.2.1.9Display the word mapDisplay the reaction diagram Show all sequences 1.2.1.995 - 10 min, complete inactivation 657250
Results 1 - 9 of 9