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EC Number	Temperature Stability Minimum [�C]	Temperature Stability Maximum [�C]	Commentary	Reference
1.2.1.59	4	-	the NADH-dependent activity of the chloroplast GAPDH is not affected by incubation at 4°C, for between 1 and 14 h, with either oxidized ADK3 (OxADK3) or ADK3-DELTACP12 at a ratio of 2:1 (dimeric ADK:tetrameric GAPDH)	763014
1.2.1.59	22	-	the purified recombinant enzyme retains more than 60% of its activity even after an incubation of 72 h at room temperature in the presence of 6 M urea	763005
1.2.1.59	43	-	upon heat-treatment, GAPDH becomes denatured and precipitated but ADK3 greatly improves its thermal stability, decreasing its aggregation and protecting its activity at 43°C. This ability is linked to the presence of the CP12-like extension of ADK3 as no protection is observed when this part is removed	763014
1.2.1.59	75	-	pH 7.0, 30 min, stable	11539
1.2.1.59	100	-	half-life of the purified recombinant enzyme is 60 min	763005
1.2.1.59	100	-	half-life: 44 min	11537

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Release 2023.1 (January 2023)