EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
    1.1.1.82 | 37 |
- |
pH 8.0, 10 min, reduced enzyme without NADPH, about 50% loss of activity |
287417 |
| 1.1.1.82 | 50 |
- |
pH 8.0, 10 min, reduced enzyme in presence of NADPH, about 70% loss of activity |
287417 |
| 1.1.1.82 | 50 |
- |
purified recombinant enzyme, 30 min, pH 5.0-10.5, stable |
696461 |
| 1.1.1.82 | 55 |
- |
pH 8.0, 10 min, oxidized enzyme without NADPH, about 75% loss of activity |
287417 |
| 1.1.1.82 | 60 |
- |
pH 8.0, 10 min, oxidized enzyme in presence of NADPH, about 50% loss of activity |
287417 |
| 1.1.1.82 | 90 |
- |
purified recombinant enzyme, 10 min, stable. The hyperthermostability of the Aeropyrum pernix MDH is likely attributable to its smaller cavity volume and larger numbers of ion pairs and ion-pair networks, but the molecular strategy for thermostability may be specific for each enzyme |
696461 |
| 1.1.1.82 | 100 |
- |
purified recombinant enzyme, 10 min, stable. The hyperthermostability of the Aeropyrum pernix MDH is likely attributable to its smaller cavity volume and larger numbers of ion pairs and ion-pair networks, but the molecular strategy for thermostability may be specific for each enzyme |
696461 |
