EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
1.1.1.121 | 46 |
- |
half-life: 1 min, biphasic inactivation curve |
285797 |
1.1.1.121 | 50 |
- |
rapidly inactivated |
285790 |
1.1.1.121 | 55 |
- |
half-life: 40 s in sodium phosphate buffer, pH 7.0, with D-fucose, D-galactose, D-glucose or D-mannose as substrate |
285794 |
1.1.1.121 | 60 |
- |
stable up to, pH 7.5, 15 min |
719111 |
1.1.1.121 | 80 |
- |
mutant enzyme D242N loses about 20% of activity |
747502 |
1.1.1.121 | 85 |
- |
15 min, wild-type enzyme is stable, mutant enzyme F141A loses about 5% of activity, mutant enzyme F249A loses about 10% of activity, mutant enzyme D242N loses 60% of activity |
747502 |
1.1.1.121 | 85 |
- |
approximately 40% of the activity is inactivated by the incubation of the enzyme solution (0.3 mg/ml in 10 mM potassium phosphate, pH 7.0) for 15 min at 95°C |
697263 |
1.1.1.121 | 90 |
- |
15 min, wild-type enzyme loses about 10% of its activity, mutant enzyme F141A loses about 25% of activity, mutant enzyme F249A loses about 25% of activity, mutant enzyme D242N completely loses activity |
747502 |
1.1.1.121 | 95 |
- |
15 min, wild-type enzyme loses about 40% of its activity, mutant enzyme F141A loses about 50% of activity, mutant enzyme F249A loses about 70% of activity, mutant enzyme D242N completely loses activity |
747502 |