EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
3.4.23.18 | 20 |
25 |
pH 2.7, 16 h stable |
30564 |
3.4.23.18 | 20 |
45 |
after incubation at 20°C for 1 h, the enzyme with propeptide retains 100% activity. 38.1°C is the melting temperature of the catalytically active enzyme with propeptide. When the temperature is over 45°C, the relative enzyme activities of the enzyme with propeptide decreases to below 40% |
755227 |
3.4.23.18 | 30 |
- |
pH 7, drastic decrease of activity probably due to autolytic activity |
30564 |
3.4.23.18 | 30 |
50 |
the enzyme maintains 39.8% of its maximum activity after 5 min incubation at 50°C. The protease remains stable after 1 h of incubation at 30 and 35°C, retaining more than 70% activity |
753450 |
3.4.23.18 | 40 |
- |
below, stable |
30560 |
3.4.23.18 | 50 |
- |
the enzyme maintains 80% activity when incubated at 50°C and pH 3.0 for 20 min |
754494 |
3.4.23.18 | 50 |
- |
up to, stable, at pH 3.8 |
30563 |
3.4.23.18 | 50 |
80 |
the enzyme is almost 100 % stable at 50°C for 24 h. Retention of full activity is seen for over 4, 2, 1 h and 30 min at 50, 60, 70 and 80°C, respectively. However, a loss in relative activity in the range of 1.3 % (50°C) to 19.6 % (at 80°C) is noted after 24 h |
752341 |
3.4.23.18 | 55 |
- |
10 min, in 50 mM sodium acetate buffer, pH 5 |
30569, 30570, 30575 |
3.4.23.18 | 55 |
- |
20 min, complete inactivation at pH 5.5, Ca2+ does not protect |
30564 |