EC Number   |
Temperature Stability Minimum [°C]  |
Temperature Stability Maximum [°C] |
Reference |
|---|
   3.4.19.3 | -999 |
- |
alpha6-helix (from Ser188 to Glu205) of PCP-0SH is stably formed in the D1 state |
678264 |
| 3.4.19.3 | -999 |
- |
the heat denaturation of wild-type enzyme and mutant enzymes C188S and C142S/C188S is highly reversible in the dimeric forms, but completely irreversible in the tetrameric form |
651099 |
| 3.4.19.3 | -999 |
- |
the thermal stability of the mutant enzymes C142S/C188S/E192A, C142S/C188S/E192I, C142S/C188S/E192V, C142S/C188S/E192D and C142S/C188S/E192Q at pH 2.15, pH 3.04 and pH 7.3 is less than that of the cysteine-free mutant enzyme C142S/C188S. At pH 8.7 and 9.6 the thermal stability of mutant enzymes C142S/C188S/E192A, C142S/C188S/E192I, C142S/C188S/E192V and C142S/C188S/E192Q is higher than that of the cysteine-free mutant C142S/C188S. The thermal stability of mutant enzyme C142S/C188S/E192D at pH 8.7 and 9.6 is less than that of cysteine-free mutant enzyme C142S/C188S |
667733 |
| 3.4.19.3 | 7.5 |
8.5 |
stable |
667525 |
| 3.4.19.3 | 22 |
- |
room temperature, 40% loss of activity after 15 days, 57% loss of activity after 1 month |
95294 |
| 3.4.19.3 | 24 |
- |
50 mM phosphate buffer, pH 7, 10 mM 2-mercaptoethanol, 28% loss of activity after 1 month |
95303 |
| 3.4.19.3 | 30 |
- |
pH 7.0, 30 min, about 5% loss of activity of wild-type enzyme and of mutant enzyme S185C |
650352 |
| 3.4.19.3 | 37 |
- |
1 h, rapid inactivation |
95295 |
| 3.4.19.3 | 40 |
- |
4.5 h, stable up to |
653759 |
| 3.4.19.3 | 40 |
- |
pH 7, 30 min, 5 mM EDTA and 2-mercaptoethanol, stable |
95290 |
