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Results 1 - 10 of 28 > >>
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Reference
-999
-
alpha6-helix (from Ser188 to Glu205) of PCP-0SH is stably formed in the D1 state
-999
-
the heat denaturation of wild-type enzyme and mutant enzymes C188S and C142S/C188S is highly reversible in the dimeric forms, but completely irreversible in the tetrameric form
-999
-
the thermal stability of the mutant enzymes C142S/C188S/E192A, C142S/C188S/E192I, C142S/C188S/E192V, C142S/C188S/E192D and C142S/C188S/E192Q at pH 2.15, pH 3.04 and pH 7.3 is less than that of the cysteine-free mutant enzyme C142S/C188S. At pH 8.7 and 9.6 the thermal stability of mutant enzymes C142S/C188S/E192A, C142S/C188S/E192I, C142S/C188S/E192V and C142S/C188S/E192Q is higher than that of the cysteine-free mutant C142S/C188S. The thermal stability of mutant enzyme C142S/C188S/E192D at pH 8.7 and 9.6 is less than that of cysteine-free mutant enzyme C142S/C188S
7.5
8.5
stable
22
-
room temperature, 40% loss of activity after 15 days, 57% loss of activity after 1 month
24
-
50 mM phosphate buffer, pH 7, 10 mM 2-mercaptoethanol, 28% loss of activity after 1 month
30
-
pH 7.0, 30 min, about 5% loss of activity of wild-type enzyme and of mutant enzyme S185C
37
-
1 h, rapid inactivation
40
-
4.5 h, stable up to
40
-
pH 7, 30 min, 5 mM EDTA and 2-mercaptoethanol, stable
Results 1 - 10 of 28 > >>