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EC Number Cloned (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 6.3.5.6canonical set of amidotransferase genes gatA, gatB and gatC is cloned and sequenced, the holoenzyme is overexpessed in Escherichia coli and exhibits both Asp-tRNAAsn and Glu-tRNAGln transamidation activities, the gatA, gatB and gatC genes are dispersed in the genome and encode polypeptides of 472, 470 and 90 amino acids, removal of the 44 carboxy-terminal amino acids of the GatA subunit only inhibits the Asp-AdT activity, but not the Glu-AdT activity 440090
Display the word mapDisplay the reaction diagram Show all sequences 6.3.5.6cloning of the gatC, gatA and gatB genes, situated in an operon-like manner, encoding the GatCAB amidotransferase and overexpression in Escherichia coli BL21-Codon Plus-TM 440092
Display the word mapDisplay the reaction diagram Show all sequences 6.3.5.6cloning of the gatCAB genes encoding the Asp/Glu-AdT and overexpression in Escherichia coli 440095
Display the word mapDisplay the reaction diagram Show all sequences 6.3.5.6cloning of the gatCAB operon encoding the Asp-tRNAAsn amidotransferase and expression in Escherichia coli BL21-Codon Plus(DE3)-RIL 440091
Display the word mapDisplay the reaction diagram Show all sequences 6.3.5.6expressed in Escherichia coli 715924
Display the word mapDisplay the reaction diagram Show all sequences 6.3.5.6expression in Escherichia coli 675227, 746380
Display the word mapDisplay the reaction diagram Show all sequences 6.3.5.6expression in Escherichia coli strain BL21(DE3), co-expression with the cytotoxic nondiscriminating aspartyl-tRNA synthetase, EC 6.1.1.23, which is toxic for the cells, rescues the toxicity, overview 672190
Display the word mapDisplay the reaction diagram Show all sequences 6.3.5.6gatCAB genes encode Asp-AdT 440093
Display the word mapDisplay the reaction diagram Show all sequences 6.3.5.6gatCAB genes encoding Glu/Asp-AdT are cloned and expressed in Escherichia coli 440087
Display the word mapDisplay the reaction diagram Show all sequences 6.3.5.6overexpression in Escherichia coli 659057
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