EC Number |
Reference |
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6.3.5.6 | canonical set of amidotransferase genes gatA, gatB and gatC is cloned and sequenced, the holoenzyme is overexpessed in Escherichia coli and exhibits both Asp-tRNAAsn and Glu-tRNAGln transamidation activities, the gatA, gatB and gatC genes are dispersed in the genome and encode polypeptides of 472, 470 and 90 amino acids, removal of the 44 carboxy-terminal amino acids of the GatA subunit only inhibits the Asp-AdT activity, but not the Glu-AdT activity |
440090 |
6.3.5.6 | cloning of the gatC, gatA and gatB genes, situated in an operon-like manner, encoding the GatCAB amidotransferase and overexpression in Escherichia coli BL21-Codon Plus-TM |
440092 |
6.3.5.6 | cloning of the gatCAB genes encoding the Asp/Glu-AdT and overexpression in Escherichia coli |
440095 |
6.3.5.6 | cloning of the gatCAB operon encoding the Asp-tRNAAsn amidotransferase and expression in Escherichia coli BL21-Codon Plus(DE3)-RIL |
440091 |
6.3.5.6 | expressed in Escherichia coli |
715924 |
6.3.5.6 | expression in Escherichia coli |
675227, 746380 |
6.3.5.6 | expression in Escherichia coli strain BL21(DE3), co-expression with the cytotoxic nondiscriminating aspartyl-tRNA synthetase, EC 6.1.1.23, which is toxic for the cells, rescues the toxicity, overview |
672190 |
6.3.5.6 | gatCAB genes encode Asp-AdT |
440093 |
6.3.5.6 | gatCAB genes encoding Glu/Asp-AdT are cloned and expressed in Escherichia coli |
440087 |
6.3.5.6 | overexpression in Escherichia coli |
659057 |