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EC Number Cloned (Commentary)
Show all pathways known for 6.3.4.2Display the word mapDisplay the reaction diagram Show all sequences 6.3.4.2-
Show all pathways known for 6.3.4.2Display the word mapDisplay the reaction diagram Show all sequences 6.3.4.2a carboxyl-terminal FLAG sequence is introduced after insertion of human CTPS1 into pCDNA 4 myc/his, expression by transient transfection of human embryonic kidney (HEK) 293 cells
Show all pathways known for 6.3.4.2Display the word mapDisplay the reaction diagram Show all sequences 6.3.4.2cDNA encoding the synthetase domain of human CTPS (isoform 1) is subcloned into the pNIC-Bsa4 vector, the resulting construct codes for a protein with an N-terminal hexahistidine tag with an integrated TEV protease-cleavage site, the entire plasmid is transformed in Escherichia coli BL-21
Show all pathways known for 6.3.4.2Display the word mapDisplay the reaction diagram Show all sequences 6.3.4.2cloned in Escherichia coli as a histidine-tagged fusion protein
Show all pathways known for 6.3.4.2Display the word mapDisplay the reaction diagram Show all sequences 6.3.4.2cloned in Escherichia coli BL-21 as an N-terminal 6xHis-tag fusion protein
Show all pathways known for 6.3.4.2Display the word mapDisplay the reaction diagram Show all sequences 6.3.4.2expressed in Escherichia coli BL21(DE3) cells
Show all pathways known for 6.3.4.2Display the word mapDisplay the reaction diagram Show all sequences 6.3.4.2expression in a ura7ura8 double mutant that lacks CTP synthetase activity
Show all pathways known for 6.3.4.2Display the word mapDisplay the reaction diagram Show all sequences 6.3.4.2expression in Escherichia coli
Show all pathways known for 6.3.4.2Display the word mapDisplay the reaction diagram Show all sequences 6.3.4.2expression of the human type II CTP synthetase gene in the CTP synthetase-deficient mutant Escherichia coli JF618 completely abolishes the need for exogenous cytidine for their survival
Show all pathways known for 6.3.4.2Display the word mapDisplay the reaction diagram Show all sequences 6.3.4.2expression of the mutant enzyme D149E in Escherichia coli
Results 1 - 10 of 19 > >>