EC Number   |
Reference  |
|---|
   6.3.2.30 | expression in Escherichia coli TOP10 cells |
660739, 660741, 660829 |
| 6.3.2.30 | expression in Escherichia coli DH5alpha |
661619 |
| 6.3.2.30 | expression in Escherichia coli BL21(DE3) |
661623 |
| 6.3.2.30 | expression in Escherichia coli DH1 cells |
662783 |
| 6.3.2.30 | engineered cyanophycin synthetase (CphA) from Nostoc ellipsosporum confers enhanced CphA activity and cyanophycin accumulation to Escherichia coli |
671478 |
| 6.3.2.30 | gene cphA6308, functional expression in Saccharomyces cerevisiae strains G175 and BY4741, which is much more efficient with the copper ion-inducible CUP1 promoter instead of the GAL1 promoter, the yeast strains produce water-soluble and water-insoluble cyanophycin polymer. Growth of transgenic yeasts in the presence of 15 mM lysine results in an incorporation of up to 10 mol% of lysine into cyanophycin, overview. Subcloning in Escherichia coli strain XL1-Blue |
690533 |
| 6.3.2.30 | gene cphA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional overexpression of His-tagged Tlr2170 in Escherichia coli BL21(DE3) |
690592 |
| 6.3.2.30 | gene cphA, subcloning and expression of His-tagged wild-type and mutant enzymes in Escherichia coli strains DH5alpha and BL21(DE3) |
691115 |
| 6.3.2.30 | functional expression in Nicotiana tabacum var. Petit Havana SRI targeted to the chloroplasts using the CaMV 35S promoter and a translocation pathway signal sequence, the phenotypic abnormalities are reduced by this way, cyanophycin accumulation in chloroplasts, overview |
694618 |
| 6.3.2.30 | gene cphA6308, expression in Pichia pastoris and Escherichia coli from expression vectors pPICHOLI-C with the copper-inducible CUP1 promoter and pPICHOLI-3 with the methanol-inducible AOX1 promoter. Stabilization of the expression plasmid, the his4 gene from Saccharomyces cerevisiae is introduced and the expression is made in the His auxotrophic Pichia pastoris strain GS115. Recombinant polymer production by wild-type and mutant enzymes and product compositions, overview |
701820 |
