EC Number   |
|---|
    6.3.2.3 | - |
| 6.3.2.3 | cloning and amplification of a gene for glutathione synthetase |
| 6.3.2.3 | cloning and complementation of a gsh2 mutant in fission yeast |
| 6.3.2.3 | cloning and sequencing of the large subunit |
| 6.3.2.3 | cloning of the 2.2 kb 5'-flanking region, DNA sequence determination and analysis, promotor determination, determination of positive and negative regulation regions, e.g. NF1 repressor for induction of enzyme expression by tert-butylhydroquinone, expression in H4IIE cells, ATCC CRL-1548, via transformation by recombinant adenovirus vector |
| 6.3.2.3 | cloning of the enzyme promoter and molecular mechanisms of GS transcriptional regulation, overview. Nrf1 and Nrf2 overexpression induces the human GS promoter activity. Human GS promoter contains two regions with homology to the nuclear factor erythroid 2, NFE2, motif that are required for basal activity as mutation of these sites reduces the human GS promoter activity by 66% |
| 6.3.2.3 | cloning of the enzyme promoter and molecular mechanisms of GS transcriptional regulation. The rat GS promoter contains functional AP-1 sites, some of which act as enhancers. It also contains a functional NF1 site that acts as a repressor, and basal expression requires AP-1 and NFkappaB, overview |
| 6.3.2.3 | cloning of the large subunit |
| 6.3.2.3 | DNA and amino acid sequence determination |
| 6.3.2.3 | DNA sequence analysis, overexpression of gene gshs2 in an enzyme-deficient Schizosaccharomyces pombe strain, expression of wild-type enzyme, isolated enzyme subunit fragments and mutants as C-terminally His-tagged proteins, subcloning in Escherichia coli DH5alpha |
