EC Number   |
|---|
    6.2.1.26 | gene AAE14, complementates an Escherichia coli mutant disrupted in the menE gene encoding the enzyme, and restores menaquinone biosynthesis |
| 6.2.1.26 | gene menE, DNA and amino acid sequence determination and analysis, expression of the His6-tagged enzyme in Escherichia coli strain BL21(DE3) |
| 6.2.1.26 | isolation of two CoE1-men+ hybrid plasmids, one of which contains the entire menD gene. Alignment of the genetic and restriction site maps of the men region and the amplification of menaquinone biosynthetic enzyme activities in plasmid-carrying strains |
| 6.2.1.26 | recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) |
