EC Number |
Reference |
---|
6.2.1.16 | AACS DNa and amino acid sequence determination and analysis, and promoter identification and cloning, the human AACS promoter is a peroxisome-proliferator-activated receptor gamm, PPARgamma, target gene, the nuclear receptor is recruited to the AACS promoter by direct interaction with stimulating protein-1, Sp-1 |
702149 |
6.2.1.16 | DNA and amino acid sequence determination, expression in Escherichia coli JM109, the engineered strain utilizes acetoacetate and can synthesize carotenoids, e.g. alpha-humulene, effectively, overview |
690593 |
6.2.1.16 | expressed in Escherichia coli |
653080 |
6.2.1.16 | expression of a cDNA isolated from human carcinoma HepG2 in Escherichia coli |
649800 |
6.2.1.16 | gene AACS, analysis of the transcriptional mechanism of AACS in Neuro-2a neuroblastoma cells. The minimal core promoter of the mouse AACS gene is located in a region with 110 bps upstream from the transcription start site. Mutagenesis studies show that the Sp1 binding site is crucial for AACS promoter activity. Sp1 binds to the Sp1 binding site on the promoter region of AACS. Overexpression of Sp1 increases AACS mRNA levels. Real-time PCR enzyme expression analysis. Functional analysis of the 5'-flanking region of AACS in Neuro-2a cells |
744249 |
6.2.1.16 | gene Aacs, cloning and recombinant expression of FLAG-tagged enzyme in Lenti-X-293T cells. AACS and legumain are transiently expressed in HEK-293 cells |
744226 |
6.2.1.16 | gene aacS, expression in Escherichia coli and complementation of a deficiency in atoDA, encoding acetyl-CoA:acetotacetate CoA transferase, EC 2.8.3.8, in Escherichia coli strain DELtaatoDA DELTAcobB DELTApka |
728335 |
6.2.1.16 | gene Aacs, recombinant expression of FLAG-tagged enzyme in Lenti-X-293T cells, overexpression of wild-type and mutant enzymes in HEK-293 cells |
744906 |
6.2.1.16 | recombinant enzyme expression |
746014 |