EC Number   |
|---|
    5.4.3.2 | - |
| 5.4.3.2 | expressed in Escherichia coli |
| 5.4.3.2 | expression Escherichia coli |
| 5.4.3.2 | expression in Escherichia coli |
| 5.4.3.2 | gene ablA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21(DE3) |
| 5.4.3.2 | gene kamA, DNA and amino acid sequence determination and analysis, sequence analysis of the kam locus, genetic organization, the kam locus forms two operons: yodT (yodT-yodS-yodR-yodQ-yodPkamR) and kamA (kamA-yokU-yozE). The transcriptional start sites (TSSs) of the kamA gene were determined using 5' rapid amplification of cDNA ends (RACE). A typical -12/-24 sigma54 binding site is identified in the promoter PkamA, which is located upstream of the kamA gene TSS. PkamA, which directs the transcription of the kamA operon, is controlled by the sigma54 factor and is activated through the sigma54-dependent transcriptional regulator KamR. The kamA operon is also controlled by sigmaK and regulated by the GerE protein in the late stage of sporulation |
| 5.4.3.2 | gene kamA, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS |
| 5.4.3.2 | gene yjeK, construction of polycistronic vectors encoding His-tagged or non-tagged EF-P, with YjeA, a class II lysyl-tRNA synthetase, and YjeK and overexpression in Escherichia coli strain BL21(DE3) |
