EC Number |
---|
5.3.99.5 | - |
5.3.99.5 | characterization of the gene TBXAX1 encoding thromboxane synthase |
5.3.99.5 | expression in Escherichia coli |
5.3.99.5 | expression of C-terminally His-tagged enzyme replacing the first 29 amino acid residues in the N-terminal transmembrane domain with the hydrophilic sequence MAKKTSS for TXAS cDNA in Escherichia coli strain DH5alpha |
5.3.99.5 | expression of wild-type and mutant C-terminally His4-tagged enzyme replacing the first 28 amino acid residues in the N-terminal transmembrane domain with the hydrophilic sequence MAKKTSS for TXAS cDNA in Escherichia coli strain BL21/(DE3)pLys |
5.3.99.5 | overexpression in Escherichia coli |
5.3.99.5 | stable overexpression of thromboxane synthase in SKMES-1 cells |