EC Number   |
Reference |
|---|
    5.1.3.6 | expression in Agrobacterium tumefaciens |
700488 |
| 5.1.3.6 | expression in Escherichia coli |
704357 |
| 5.1.3.6 | expression in Pichia pastoris |
661739, 663125 |
| 5.1.3.6 | gene UGlcAE3, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, real-time quantitative PCR enzyme expression analysis, recombinant expression in Pichia pastoris strain GS115 in the microsomal fraction. The recombinant OcUGlcAE3 can form a microsomal protein by the N-terminal membrane anchor sequence, or can be secrete into the extracellular space through the guidance of the signal peptide. OcUGlcAE3 can be expressed in Escherichia coli strain Transetta (DE3) only as truncated enzyme with deletion of a putative transmembrane domain of 128 aa in the N-terminus |
748715 |
| 5.1.3.6 | isoform GAE3 is expressed in Escherichia coli BL21(DE3) cells |
716216 |
| 5.1.3.6 | isoform GAE6 is expressed in Escherichia coli BL21(DE3) cells |
716216 |
| 5.1.3.6 | no recovery of active recombinant enzyme when full-length AtUGlcAE1 is expressed in Escherichia coli due to the hydrophobic domain that results in un- or misfolded protein in inclusion bodies within Escherichia coli. Recombinant AtUGlcAE1DELTA1-64, lacking the putative transmembrane domain, can be expressed in Escherichia coli |
663134 |
| 5.1.3.6 | overexpression in Escherichia coli |
653259 |
| 5.1.3.6 | recombinant ZmUGlcAE, lacking the putative transmembrane domain at amino acid position 1-47, is expressed in Escherichia coli |
704357 |
