EC Number   |
Reference |
|---|
    5.1.3.3 | - |
684574 |
| 5.1.3.3 | baculovirus expression system |
661785 |
| 5.1.3.3 | cloned into pRSET-C vector (Invitrogen), expression in Escherichia coli |
671057 |
| 5.1.3.3 | construction of a shuttle plasmid for Bacillus megaterium and Escherichia coli that contains the promoter and repressor gene of the Bacillus megaterium borne operon for xylose utilization. A polylinker downstream the promoter allows versatile cloning of genes under its transcriptional control |
2361, 2378 |
| 5.1.3.3 | efficient stereospecific enzymatic synthesis of L-[15N]-valine, L-[15N]-leucine, L-[15N]-norvaline, L-[15N]-norleucine and L-[15N]-isoleucine from the corresponding alpha-keto acids by coupling the reactions catalysed by leucine dehydrogenase and glucose dehydrogenase/galactose mutarotase. Giving high yields of L-amino acids, the procedure is economical and easy to perform and to monitor at a synthetically useful scale of 1-10 g. Substrates alpha-ketoisovalerate, alpha-ketoisocaproate, alpha-ketovalerate, alpha-ketocaproateand alpha-keto-beta-methylvalerate are converted to products L-valine, L-leucine, L-norvaline, L-isoleucine with yields of 90%, 92.5%, 92%, 80%, and 95%, respectively |
693504 |
| 5.1.3.3 | expressed in Escherichia coli Rosetta (DE3) cells |
713653 |
| 5.1.3.3 | expression in Corynebacterium glutamicum |
660749 |
| 5.1.3.3 | expression in Escherichia coli |
2353, 2361, 651324, 673761 |
| 5.1.3.3 | expression of the C-terminal galactose mutarotase part of Gal10 in Hypocrea jecorina |
694901 |
| 5.1.3.3 | from genomic DNA |
662113 |
