EC Number   |
|---|
   5.1.3.17 | - |
| 5.1.3.17 | cloned into a baculovirus expression vector and expressed in Sf9 insect cells |
| 5.1.3.17 | DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic tree, recombinant expression of soluble FLAG-tagged enzyme mutant lacking the transmembrane domain (N-terminal 33 amino acids) in insect cells using the baculovirus expression system |
| 5.1.3.17 | enzyme GLCE is ectopically re-expressed in morphologically different LNCaP and PC3 prostate cancer cells, quantitative real-time PCR enzyme expression analysis |
| 5.1.3.17 | expressed in U2020 cells |
| 5.1.3.17 | expression of wild-type and mutant enzymes in Escherichia coli strain DE3 |
| 5.1.3.17 | gene glce, recombinant expression of enzyme mutant comprising residues Arg50-Asn585 as N-terminally His6-SUMO-tagged enzyme in Escherichia coli strain BL21(DE3), expression of N-terminally His6-SUMO-tagged SeMet-substituted Glce protein in Escherichia coli strain B834, expression of diverse enzyme point mutants |
| 5.1.3.17 | gene Hsepi |
| 5.1.3.17 | gene RED65_08024, design and synthesis of a synthetic based on hypothetical protein RED65_08024, codon optimized for the codon usage bias of Escherichia coli, expression of the recombinant His-tagged enzyme in Escherichia coli strain Rossetta(DE3)/pWUR537 |
| 5.1.3.17 | recombinant expression in Escherichia coli |
