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EC Number Cloned (Commentary)
Display the word mapDisplay the reaction diagram Show all sequences 5.1.1.17expression in Penicillium chrysogenum
Display the word mapDisplay the reaction diagram Show all sequences 5.1.1.17Penecillium chrysogenum strains are constructed expressing cefD1 (isopenecillin N-CoA synthetase), cefD2 (isopenecillin N-CoA epimerase), cefEF (deacetylcephalosporin-actyltransferase), and cefG (deacetylcephalosporin C synthetase) genes cloned from Acremonium chrysogenum. HPLC analysis of cell extracts show that transformant TA64, TA71, and TA98 accumulate intracellularly deacetylcephalosporin C and, in the last strain (TA98), also cephalosporin C.
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