Any feedback?
Please rate this page
(search_result.php)
(0/150)

BRENDA support

Refine search

Search Cloned(Commentary)

show results
Don't show organism specific information (fast!)
Search organism in taxonomic tree (slow, choose "exact" as search mode, e.g. "mammalia" for rat,human,monkey,...)
(Not possible to combine with the first option)
Refine your search

Search term:

Results 1 - 10 of 13 > >>
EC Number Cloned (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 4.3.1.7- 713644
Display the word mapDisplay the reaction diagram Show all sequences 4.3.1.7cloning and overexpression of three different versions (eut-LpET151 his-tagged version (appends a cleavable 33-amino acid tag sequence to the N-terminus), Eut-L_NHIS (short His6-tagged version) and Eut-L_CHIS (short His6-tagged version)) of the protein is carried out directly from the Escherichia coli genome by selective PCR amplification. Selenomethione-derivatized proteins are obtained by growing cloned bacteria in selenomethionine-containing M9 minimal media. Protein overexpression and purification are performed. 690280
Display the word mapDisplay the reaction diagram Show all sequences 4.3.1.7cloning in Escherichia coli overexpression strain incorporating the cloned Salmonella typhimurium EAL coding sequence 691337
Display the word mapDisplay the reaction diagram Show all sequences 4.3.1.7Escherichia coli overexpression strain incorporating the cloned Salmonella typhimurium EAL coding sequence 691009
Display the word mapDisplay the reaction diagram Show all sequences 4.3.1.7expressed as a His-taggd fusion protein 729228
Display the word mapDisplay the reaction diagram Show all sequences 4.3.1.7expressed in Escherichia coli as a His-tagged fusion protein 729253
Display the word mapDisplay the reaction diagram Show all sequences 4.3.1.7expression in Escherichia coli 704362, 714261
Display the word mapDisplay the reaction diagram Show all sequences 4.3.1.7expression in Escherichia coli overexpression strain incorporating the cloned Salmonella typhimurium EAL coding sequences 692764
Display the word mapDisplay the reaction diagram Show all sequences 4.3.1.7for expression in Escherichia coli 680355
Display the word mapDisplay the reaction diagram Show all sequences 4.3.1.7N-terminal His6-tagged beta subunit lacking residues Lysbeta4-Cysbeta43 715530
Results 1 - 10 of 13 > >>