EC Number   |
|---|
   4.3.1.7 | - |
| 4.3.1.7 | cloning and overexpression of three different versions (eut-LpET151 his-tagged version (appends a cleavable 33-amino acid tag sequence to the N-terminus), Eut-L_NHIS (short His6-tagged version) and Eut-L_CHIS (short His6-tagged version)) of the protein is carried out directly from the Escherichia coli genome by selective PCR amplification. Selenomethione-derivatized proteins are obtained by growing cloned bacteria in selenomethionine-containing M9 minimal media. Protein overexpression and purification are performed. |
| 4.3.1.7 | cloning in Escherichia coli overexpression strain incorporating the cloned Salmonella typhimurium EAL coding sequence |
| 4.3.1.7 | Escherichia coli overexpression strain incorporating the cloned Salmonella typhimurium EAL coding sequence |
| 4.3.1.7 | expressed as a His-taggd fusion protein |
| 4.3.1.7 | expressed in Escherichia coli as a His-tagged fusion protein |
| 4.3.1.7 | expression in Escherichia coli |
| 4.3.1.7 | expression in Escherichia coli overexpression strain incorporating the cloned Salmonella typhimurium EAL coding sequences |
| 4.3.1.7 | for expression in Escherichia coli |
| 4.3.1.7 | N-terminal His6-tagged beta subunit lacking residues Lysbeta4-Cysbeta43 |
