EC Number   |
Reference |
|---|
   4.2.1.30 | - |
651642, 747370 |
| 4.2.1.30 | 1,3-propanediol operon of dhaB1 (encodes the vitamin B12-independent glycerol dehydratase), dhaB2 (encodes the activating factor of DhaB1) tandemly arrayed with Escherichia coli yqhD gene (encodes 1,3-propanediol oxidoreductase) under the control of a constitutive, temperature-sensitive promoter, in the vector pBV220 for heterologous expression in Escherichia coli K-12 ER2925 |
695764 |
| 4.2.1.30 | a synthetic pathway 1,3-propanediol production pathway is introduced in recombinant Escherichia coli consisting of glycerol dehydratase complex (dhaB123) and glycerol dehydratase reactivation factors (gdrAB) from Klebsiella pneumoniae and 1,3-propanediol oxidoreductase isoenzyme (yqhD) from Escherichia coli |
747360 |
| 4.2.1.30 | cloning of B12-independent glycerol dehydratase and its reactivase from Clostridium butyricum VPI1718, and expression in Escherichia coli BL21 (DE3). The activity of glycerol dehydratase activity is six times higher than in Clostridium butyricum |
747653 |
| 4.2.1.30 | expressed in Escherichia coli JM109 cells |
725951 |
| 4.2.1.30 | expressed in Escherichia coli XL10-Gold cells |
729463 |
| 4.2.1.30 | expression in Escherichia coli |
33834, 33835, 33837, 672728, 673100, 675575, 675901 |
| 4.2.1.30 | expression in Escherichia coli, which does not possess the 3-hydroxypropionic acid biosynthetic pathway |
748579 |
| 4.2.1.30 | expression of the dhaB gene in Escherichia coli BL21(DE3) |
748257 |
| 4.2.1.30 | genes dhaBCE, recombinant expression of His6-tagged GDHt in Escherichia coli strain Bl21(DE3), optimization of IPTG inducting process for recombinant GDHt to increase specific activity and ratio of soluble form using 0.8 mM IPTG at 20°C for 3 h, method development, overview |
713813 |
