EC Number   |
|---|
   4.1.1.35 | an Escherichia coli system containing the sugar biosynthetic pathway and the glycosyltransferase gene from Arabidopsis thaliana for the production of xylosylated naringenin is generated, Escherichia coli XL1-Blue (MRF) is used as a host cell for recombinant plasmid preparation and DNA manipulation, whereas Escherichia coli BL21 (DE3) DELTApgi is used for biotransformation of the recombinant |
| 4.1.1.35 | cDNA cloned and expressed in Escherichia coli, clone PsUXS1 |
| 4.1.1.35 | cloned and expressed in bacteria and transfected HEK293T cells |
| 4.1.1.35 | expressed in Escherichia coli |
| 4.1.1.35 | expressed in Escherichia coli BL21 (DE3) pLysS cells |
| 4.1.1.35 | expressed in Escherichia coli BL21(DE3) cells |
| 4.1.1.35 | expression in Escherichia coli |
| 4.1.1.35 | expression in Escherichia coli BL21 (DE3), the deoxysugar biosynthesis of Streptomyces sp. KCTC 0041BP is inactivated by gene replacement to generate Streptomyces sp. GerSM2 mutant, which is unable to produce dihydrochalcomycin, calS9 UDP-xylose biosynthetic genes are cloned in an integrative plasmid pSET152 to generate pBPDS, which is heterologously expressed in Streptomyces sp. GerSM2. A glycosylated product, a 5-O-xylosyl-chalconolide derivative, in the conjugal transformants is isolated |
| 4.1.1.35 | expression in Escherichia coli BL21 as His-tagged enzyme |
| 4.1.1.35 | expression of HvUXS1 cDNA in Escherichia coli |
