3.5.1.60 | gene NAAA, genotyping, six splice variants (a1, b1, c1, a2, b2, and c2), whereof four major splice variants (a1, a2, b2, and c2) are identified in a human prostate cancer cell line LNCaP, they are composed of exons 1-11, exons 1-10 and 12, exons 1-9 and 12, and exons 1-8 and 12, respectively, quantitative PCR expression analysis, method development for individual quantification. Variants a1 and a2 encode the same full-length NAAA protein, which is catalytically active, while b2 and c2 are translated to C-terminally truncated proteins. Recombinant expression in HEK-293 cells. The truncated forms are detected as catalytically inactive precursor proteins, but not as mature forms, expression of N-terminally FLAG-tagged isoform A of human NAAA, corresponding to splice variant a1, in HEK-293 cells. The mature enzyme form loses the N-terminal FLAG tag |