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EC Number Cloned (Commentary) Reference
Display the word mapDisplay the reaction diagram Show all sequences 3.4.23.B2cloned directly from proviral DNA of the infectious viral stock SIVmac251-32H(11/88 pool). SIVmac251-32H proteinase and ist flanking pol sequences are expressed in Escherichia coli as a fusion protein with most of the T7 bacteriophage gene 10 protein. The expressed protein forms cytoplasmic inclusion bodies which are solubilized in 8 M urea, and the recombinant enzyme is refolded, yielding active self-processing enzyme 647807
Display the word mapDisplay the reaction diagram Show all sequences 3.4.23.B2expressed in Escherichia coli with a recombinant expression system 647814
Display the word mapDisplay the reaction diagram Show all sequences 3.4.23.B2expression in Escherichia coli 647806
Display the word mapDisplay the reaction diagram Show all sequences 3.4.23.B2expression in Escherichia coli, expression as N-terminally capped, biotin-labeled precursor 668793
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