Any feedback?
Please rate this page
(search_result.php)
(0/150)

BRENDA support

Refine search

Search Cloned(Commentary)

show results
Don't show organism specific information (fast!)
Search organism in taxonomic tree (slow, choose "exact" as search mode, e.g. "mammalia" for rat,human,monkey,...)
(Not possible to combine with the first option)
Refine your search

Search term:

Results 1 - 8 of 8
EC Number Cloned (Commentary) Reference
Display the reaction diagram Show all sequences 3.4.23.50213 amino acids of the 3'-end of the HERV-K protease open reading frame are expressed in Escherichia coli. Autocatalytic cleavage of the expressed polypeptide results in a catalytically active 18200 Da protein 646017
Display the reaction diagram Show all sequences 3.4.23.50cloning of HML-2 Pro including self-processing sites and in-frame flanking sequence, HML-2 Pro is prokaryotically expressed. HML-2 Pro self-processes from the precursor during the expression, purification, and renaturation steps. Coexpression of the enzyme with HA-tagged potential human substrate proteins HSPA90AA1, MAP2K2, and C15orf57 in HEK-293 cells. The HEK293T cells harvested after 5 h do not show evidence of processing of candidate proteins due to apoptotic processes 754663
Display the reaction diagram Show all sequences 3.4.23.50expression of a full-length and C-terminally truncated enzyme in Escherichia coli 646018
Display the reaction diagram Show all sequences 3.4.23.50nucleotide sequence determination and analysis, detailed phylogenetic analysis of virus from primates and humans, and structural analysis of 100 HERV-K(HML-5) provirus sequences, reconstruction of a coding-competent HML-5 provirus, overview 670076
Display the reaction diagram Show all sequences 3.4.23.50sequence determination and analysis, expression of wild-type and C-terminally truncated mutant enzymes in Escherichia coli 668800
Display the reaction diagram Show all sequences 3.4.23.50targeting of the HERV-K PR to protease-deficient HIV-1 virions by expressing it as a Vpr fusion partner. The Vpr fusion proteins are sucessfully delivered to the HIV-1 virions, where the HERV-K PR not only autoprocesses itself to ist mature form, but also cleaves a number of HIV-1 polyproteins 646019
Display the reaction diagram Show all sequences 3.4.23.50the enzyme is expressed either as a full-length native protein or as truncated protein in Escherichia coli 646020
Display the reaction diagram Show all sequences 3.4.23.50the HERV-K113 sequence is cloned into a small plasmid vector. It is shown that based on a substantial LTR-promoter activity, full length messenger RNA and spliced env-, rec- and 1.5 kb (hel)-transcripts are produced. Envelope protein of HERV-K113 is synthesized as an 85 kDa precursor that is found partially processed. The accessory Rec protein is highly expressed and accumulates in the nucleus. Expression analysis reveals synthesis of the Gag precursor and the protease in lysates of transfected HEK-293T cells. The cloned HERV-K113 provirus is not replication competent 682977
Results 1 - 8 of 8