EC Number   |
|---|
   3.4.22.46 | - |
| 3.4.22.46 | expressed in Escherichia coli BL-21 |
| 3.4.22.46 | expressed in Escherichia coli BL21(DE3)LysE cells |
| 3.4.22.46 | expressed in Escherichia coli BL21(DE3)pLysS cells |
| 3.4.22.46 | expressed in Lactococcus lactis strain LAC71 and in Escherichia coli BL21 (DE3) cells |
| 3.4.22.46 | expression of enzyme mutants in Escherichia coli |
| 3.4.22.46 | expression of FLAG- and HA-tagged FMDV A24-L1123 mutant enzyme in LF-BK cells. T7 RNA transcripts of SpeI-digested pA24-WT, pA24-L1123 and pA24-L tag mutants, as well as transcript of control plasmid pL, are translated in vitro in rabbit reticulocyte lysates. All synthesized L mutant proteins are able to cleave themselves from the precursor L-Vp4-Vp2', the Lbeta band is absent in all L mutant translation reactions. The tn insertion in these mutant L plasmids forces translation initiation in vitro from other than the second AUG codon |
| 3.4.22.46 | expression of GST-tagged enzyme in Escherichia coli strain JM101 |
| 3.4.22.46 | expression of wild-type and mutant enzymes by in vitro transcription and translation using rabbit reticulocyte lysate |
| 3.4.22.46 | PK-15 cells are transfected with a HA-tagged Labpro expression construct, together with a luciferase reporter plasmid with the porcine IFN-alpha1 promoter or the IFN-beta promoter and pRL-TK. At 24 h post-transfection, the cells were further transfected or mock-transfected with poly(I:C) followed by the dual-luciferase assay, quantitative real-time RT-PCR, overview |
