EC Number |
---|
3.4.22.45 | - |
3.4.22.45 | 14 isolates of turnip mosaic virus, RNA extraction, reverse transctiption, amplification using RT-PCR, cloned into pGEM-T vector for sequence analysis and phylogenetic analysis. HC-Pro genes comprise 1374 nucleotides encoding a polypeptide of 458 amino acids |
3.4.22.45 | 42 isolates of plum pox virus (different types of strains. plum pox virus-D, plum pox virus-M, plum pox virus-Rec). Single-strand conformation polymorphism analysis and low-stringency single specific primer PCR analysis are performed on the HC-Pro region of the genome for genotyping |
3.4.22.45 | as P1/HC-Pro, expressed in Nicotiana benthamiana |
3.4.22.45 | binary constructs for overexpression of helper component proteinase HC-Pro in Tobacco etch virus generated, overexpression in Nicotina benthamiana via infiltration with Agrobacterium tumefaciens shown, binary vector pBin19-GFP used, pHannibal vector used for GFP-silencing inverted-repeat constructs |
3.4.22.45 | expressed in Arabidopsis |
3.4.22.45 | expressed in Escherichia coli |
3.4.22.45 | expressed in Escherichia coli and Nicotiana tabacum |
3.4.22.45 | expressed in Escherichia coli JM109 (DE3) cells |
3.4.22.45 | expressed in Escherichia coli, transformation into Agrobacterium tumefaciens C58C1 by using the binary plant expression vector pBIN61Sa |