EC Number   |
|---|
   3.4.21.74 | Bothrops atrox, moojeni |
| 3.4.21.74 | cDNA cloning |
| 3.4.21.74 | cDNA encoding ancrod synthesized with a yeast bias codon and inserted into the eukaryotic expression vector pPIC9, subsequently expressed in Pichia pastoris strain GS115 |
| 3.4.21.74 | DNA and amino acid sequence determination and analysis |
| 3.4.21.74 | expression of the enzyme N-terminally fused separately to three fusion partners, NusA, GST, and TrxA, expression in Escherichia coli strain BL21-Gold (DE3). NusA is the most efficient fusion partner to improve the solubility of recombinant gloshedobin, subcloning in Escherichia coli strain JM109 |
| 3.4.21.74 | pET-r-habutobin expressed in inclusion body of Escherichia coli. Autographa californica nuclear polyhedrosis virus and the habutobin/pPSC12 plasmid co-transfected into Sf9 cells |
| 3.4.21.74 | recombinant albofibrase1 expressed in Pichia pastoris strain X-33 |
