EC Number |
Reference |
---|
3.4.21.66 | DNA and amino acid sequence determination and analysis, phylogenetic tree |
732254 |
3.4.21.66 | gene aprE, DNA and amino acid sequence determination and analysis, phylogenetic tree, recombinant expression of wild-type and mutant N-terminally maltose-binding protein-tagged and C-terminally His6-tagged enzymes in Escherichia coli strain C43(DE3) |
731179 |
3.4.21.66 | recombinant expression in Lactococcus lactis strain P170 from vector pAMJ2008 and in strain MG1363, secretion of stable thermitase in an auto-induced fermentation setup at 30°C. The enzyme accumulates in the culture supernatant during batch fermentations and is easily activated at 50°C or by prolonged dialysis |
732871 |