EC Number   |
Reference |
|---|
   3.4.17.12 | - |
647253 |
| 3.4.17.12 | CPM cDNAs are cloned into pcDNA3 or pcDNA6 for expression in mammalian cells, to express N-terminal CFP- or YFP-tagged CPM pECFP-C1 and pEYFP-C1 vectors are used, to generate a fusion protein of CPM attached to the extracellular N-terminus of B1R pcDNA3 and 6 vectors are used |
683685 |
| 3.4.17.12 | DNA and amino acid sequence determination and analysis, genetic structure, localization on chromosome 12q13-q15, expression in insect cells via baculovirus transfection |
668817 |
| 3.4.17.12 | expressed in Escherichia coli strain DH5alpha |
696990 |
| 3.4.17.12 | expressed in HEK-293 cells, in Pichia pastoris, and in baculovirus-infected insect cells |
697395 |
| 3.4.17.12 | expression in Pichia pastoris |
668124 |
| 3.4.17.12 | expression of 3'-truncated, glycosylphosphatidylinositol-free, soluble enzyme in insect cells using the baculovirus transfection system |
669830 |
| 3.4.17.12 | expression of wild-type and mutant enzymes in COS-7 cells or HEK-293 cells. The wild-type and S406A and S406T mutants are expressed on the plasma membrane in glycosylphosphatidylinositol-anchored form, the S406P mutant is notz and is retained in a perinuclear location. Expression in baculovirus infected cells in a glycophosphatidyl-anchored form, whereas a truncated form, lacking the putative signal sequence for glycosylphosphatidyl anchoring is secreted at high levels into the medium. Both forms have lower molecular masses than native placental enzyme indicating a minimal glycosylation |
647255 |
| 3.4.17.12 | into the pCMV5, pGex4T-1 and pMalC2 vector for expression in COS-7 and Escherichia coli cells |
684055 |
| 3.4.17.12 | PCR-amplified fragment encoding hCPM is cloned into the pGEM-T-Easy cloning vector, and subsequent into the pPIC9 vector for transfomation of Pichia pastoris cells |
668124 |
