EC Number |
Reference |
---|
3.1.6.1 | - |
135531 |
3.1.6.1 | ARS-A and ARS-B, quantitative real-time RT-PCR expression analysis |
707966 |
3.1.6.1 | ARSA, cloning of the coding sequence of the human ARSA cDNA tagged with HA epitope in C-terminus into viral serotype 5 adeno-associated vector, AAV2-5 |
708658 |
3.1.6.1 | ARSA, DNA and amino acid sequence determination and analysis of wild-type and mutant |
709618 |
3.1.6.1 | astA gene from Pseudoalteromonas carrageenovora genome is subcloned into pHCEIA vector employing the hyper constitutive expression promoter from the D-amino acid aminotransferase gene of Geobacillus toebii. When the cell is cultured on fermentor containing MaxyBroth-HD medium with 1% glycerol, the enzyme activity reaches 12.8 micromol/min/mL. On MaxyBroth-HD medium with 2% glycerol, the cell shows 2.7fold higher arylsulfatase expression than that with 1% glycerol. The fed-batch cultivation employing MaxyBroth-HD medium and additional feeding of glycerol gave about 143 micromol/min/mL of arylsulfatase at 20 h, which corresponds to 4fold higher enzyme activity than that of 2% glycerol batch culture |
716072 |
3.1.6.1 | expressed in COS-7 cells |
690330 |
3.1.6.1 | expressed in Escherichia coli W3110 with pCol-MICT as the vector |
747354 |
3.1.6.1 | expressed in HT-1080 cells |
693036 |
3.1.6.1 | expression in active form in a sulfatase-deficient strain of Pseudomonas aeruginosa, thereby restoring growth on aromatic sulfates as sole sulfur source, and in Escherichia coli.Cysteine is also converted to formylglycine in Escherichia coli |
662100 |
3.1.6.1 | expression in COS-7 cells |
668717 |