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EC Number
Cloned (Commentary)
Reference
3.1.3.27
Escherichia coli strains lacking the original pgpA and/or pgpB genes
4778
,
94810
3.1.3.27
expressed in Escherichia coli
730834
3.1.3.27
expressed in Escherichia coli BL21 (DE3) cells
682752
3.1.3.27
expressed in Escherichia coli BL21 (DE3) CodonPlus RIPL cells
730029
3.1.3.27
pgpB gene is cloned into the pET22b vector (Novagen) to generate the C-terminal His6-tagged protein. Mutants were generated using standard site-directed mutagenesis. The PgpB wild type and mutant proteins are expressed in Escherichia coli strain C41(DE3)
751064
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